Yes, you can use multiple Wii Remotes with Remote Buddy concurrently 
and also map them differently. I've extensively tested it with two Wii 
Remotes. The only real limit to the number of Wii Remotes you can use 
at the same time is defined by the Bluetooth standard. So if you want 
to use more than, say, four at the same time, please keep these 
technical limitations in mind.

Best regards, 
Felix Schwarz